Video 4. HCT15 were grown in culture media depleted of calcium before being treated with FasL and filmed in similar conditions to Video 1. Absence of mature cell–cell contacts resulting from calcium depletion prevents the cell death–protective effect observed in control cells forming extensive cell–cell junctions. Indeed, FasL treatment induced cell death in all the cells independently of their position within the cell islet. Frames were taken every 15 min for 12 h.

Cell polarity and adherens junction formation inhibit epithelial Fas cell death receptor signaling

Laurent Gagnoux-Palacios, Hala Awina, Stéphane Audebert , Aurélie Rossin, Magali Mondin, Franck Borgese, Carlota Planas-Botey, Amel Mettouchi, Jean-Paul Borg, Anne-Odile Hueber 

J Cell Biol (2018) 217 (11): 3839–3852.

https://doi-org.proxy.insermbiblio.inist.fr/10.1083/jcb.201805071